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  Dual function of GTPBP6 in biogenesis and recycling of human mitochondrial ribosomes

Lavdovskaia, E., Denks, K., Nadler, F., Steube, E., Linden, A., Urlaub, H., et al. (2020). Dual function of GTPBP6 in biogenesis and recycling of human mitochondrial ribosomes. Nucleic Acids Research, 48(22), 12929-12942. doi:10.1093/nar/gkaa1132.

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Lavdovskaia, E., Author
Denks, K.1, Author              
Nadler, F., Author
Steube, E., Author
Linden, A.2, Author              
Urlaub, H.3, Author              
Rodnina, M. V.4, Author              
Richter-Dennerlein, R., Author
Affiliations:
1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
4Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: Translation and ribosome biogenesis in mitochondria require auxiliary factors that ensure rapid and accurate synthesis of mitochondrial proteins. Defects in translation are associated with oxidative phosphorylation deficiency and cause severe human diseases, but the exact roles of mitochondrial translation-associated factors are not known. Here we identify the functions of GTPBP6, a homolog of the bacterial ribosome-recycling factor HflX, in human mitochondria. Similarly to HflX, GTPBP6 facilitates the dissociation of ribosomes in vitro and in vivo. In contrast to HflX, GTPBP6 is also required for the assembly of mitochondrial ribosomes. GTPBP6 ablation leads to accumulation of late assembly intermediate(s) of the large ribosomal subunit containing ribosome biogenesis factors MTERF4, NSUN4, MALSU1 and the GTPases GTPBP5, GTPBP7 and GTPBP10. Our data show that GTPBP6 has a dual function acting in ribosome recycling and biogenesis. These findings contribute to our understanding of large ribosomal subunit assembly as well as ribosome recycling pathway in mitochondria.

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Language(s): eng - English
 Dates: 2020-12-022020-12-16
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1093/nar/gkaa1132
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 48 (22) Sequence Number: - Start / End Page: 12929 - 12942 Identifier: -