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  Allosteric transcription stimulation by RNA polymerase II super elongation complex

Chen, Y., Vos, S. M., Dienemann, C., Ninov, M., Urlaub, H., & Cramer, P. (2021). Allosteric transcription stimulation by RNA polymerase II super elongation complex. Molecular Cell, 81(16), 3386-3399.e10. doi:10.1016/j.molcel.2021.06.019.

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 Creators:
Chen, Y.1, Author           
Vos, S. M.1, Author           
Dienemann, C.1, Author           
Ninov, M.2, Author           
Urlaub, H.3, Author           
Cramer, P.1, Author           
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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 Abstract: The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we report the cryoelectron microscopy (cryo-EM) structure of ELL2-EAF1 bound to a RNA Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the RNA Pol II lobe domain, explaining how SEC delivers P-TEFb to RNA Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that the stimulation of RNA elongation requires the dimerization module and the ELL2 linker that tethers the module to the RNA Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a closed conformation of the RNA Pol II active center cleft.

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Language(s): eng - English
 Dates: 2021-07-14
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2021.06.019
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 81 (16) Sequence Number: - Start / End Page: 3386 - 3399.e10 Identifier: -