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  Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo

Zumer, K., Maier, K. C., Farnung, L., Jaeger, M. G., Rus, P., Winter, G., et al. (2021). Two distinct mechanisms of RNA polymerase II elongation stimulation in vivo. Molecular Cell, 81(15), 3096-3109.e8. doi:10.1016/j.molcel.2021.05.028.

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Genre: Journal Article

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 Creators:
Zumer, K.1, Author              
Maier, K. C.1, Author              
Farnung, L.1, Author              
Jaeger, M. G., Author
Rus, P.1, Author              
Winter, G., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: transcription; transcription elongation; RNA polymerase II; PAF1; CTR9; RTF1; SPT6; dTAG; dTAG7
 Abstract: Transcription by RNA polymerase II (RNA Pol II) relies on the elongation factors PAF1 complex (PAF), RTF1, and SPT6. Here, we use rapid factor depletion and multi-omics analysis to investigate how these elongation factors influence RNA Pol II elongation activity in human cells. Whereas depletion of PAF subunits PAF1 and CTR9 has little effect on cellular RNA synthesis, depletion of RTF1 or SPT6 strongly compromises RNA Pol II activity, albeit in fundamentally different ways. RTF1 depletion decreases RNA Pol II velocity, whereas SPT6 depletion impairs RNA Pol II progression through nucleosomes. These results show that distinct elongation factors stimulate either RNA Pol II velocity or RNA Pol II progression through chromatin in vivo. Further analysis provides evidence for two distinct barriers to early elongation: the promoter-proximal pause site and the +1 nucleosome. It emerges that the first barrier enables loading of elongation factors that are required to overcome the second and subsequent barriers to transcription.

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Language(s): eng - English
 Dates: 2021-06-182021-08-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.molcel.2021.05.028
 Degree: -

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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 81 (15) Sequence Number: - Start / End Page: 3096 - 3109.e8 Identifier: -