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  MINSTED fluorescence localization and nanoscopy

Weber, M., Leutenegger, M., Stoldt, S., Jakobs, S., Mihaila, T. S., Butkevich, A. N., et al. (2021). MINSTED fluorescence localization and nanoscopy. Nature Photonics, 15, 361-366. doi:10.1038/s41566-021-00774-2.

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 Creators:
Weber, M.1, Author              
Leutenegger, M.2, Author              
Stoldt, S.3, Author              
Jakobs, S.3, Author              
Mihaila, T. S.1, Author              
Butkevich, A. N., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
3Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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Free keywords: Super-resolution microscopy
 Abstract: We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background and the required number of fluorescence detections are low compared with most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200–1,000 detections per fluorophore provide a localization precision of 1–3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement in far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.

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Language(s): eng - English
 Dates: 2021-03-15
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41566-021-00774-2
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Title: Nature Photonics
Source Genre: Journal
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Pages: - Volume / Issue: 15 Sequence Number: - Start / End Page: 361 - 366 Identifier: -