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  Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels

Weber, M., Khan, T. A., Patalag, L. J., Bossi, M., Leutenegger, M., Belov, V. N., et al. (2021). Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels. Chemistry - A European Journal, 27(1), 451-458. doi:10.1002/chem.202004645.

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 Creators:
Weber, M.1, Author              
Khan, T. A.1, Author              
Patalag, L. J.1, Author              
Bossi, M., Author
Leutenegger, M.2, Author              
Belov, V. N.2, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: The use of photoactivatable dyes in STED microscopy has so far been limited by two-photon activation through the STED beam and by the fact that photoactivatable dyes are poorly solvable in water. Herein, we report ONB-2SiR, a fluorophore that can be both photoactivated in the UV and specifically de-excited by STED at 775 nm. Likewise, we introduce a conjugation and purification protocol to effectively label primary and secondary antibodies with moderately water-soluble dyes. Greatly reducing dye aggregation, our technique provides a defined and tunable degree of labeling, and improves the imaging performance of dye conjugates in general.

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Language(s): eng - English
 Dates: 2020-10-232021-01-04
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1002/chem.202004645
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Title: Chemistry - A European Journal
Source Genre: Journal
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Pages: - Volume / Issue: 27 (1) Sequence Number: - Start / End Page: 451 - 458 Identifier: -