English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A quantitative map of nuclear pore assembly reveals two distinct mechanisms

Otsuka, S., Tempkin, J. O. B., Politi, A. Z., Rybina, A., Hossain, M. J., Kueblbeck, M., et al. (2021). A quantitative map of nuclear pore assembly reveals two distinct mechanisms. bioRxiv, 444137. doi:10.1101/2021.05.17.444137.

Item is

Files

show Files
hide Files
:
3332258.pdf (Preprint), 3MB
Name:
3332258.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Otsuka, S., Author
Tempkin, J. O. B., Author
Politi, A. Z.1, Author              
Rybina, A., Author
Hossain, M. J., Author
Kueblbeck, M., Author
Callegari, A., Author
Koch, B., Author
Sali, A., Author
Ellenberg, J., Author
Affiliations:
1Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society, ou_2640691              

Content

show
hide
Free keywords: -
 Abstract: Understanding how the nuclear pore complex (NPC) assembles is of fundamental importance to grasp the mechanisms behind its essential function and understand its role during evolution of eukaryotes1–4. While we know that at least two NPC assembly pathways exist, one during exit from mitosis and one during nuclear growth in interphase, we currently lack a quantitative map of their molecular events. Here, we use fluorescence correlation spectroscopy (FCS) calibrated live imaging of endogenously fluorescently-tagged nucleoporins to map the changes in composition and stoichiometry of seven major modules of the human NPC during its assembly in single dividing cells. This systematic quantitative map reveals that the two assembly pathways employ strikingly different molecular mechanisms, inverting the order of addition of two large structural components, the central ring complex and nuclear filaments. Our dynamic stoichiometry data allows us to perform the first computational simulation that predicts the structure of postmitotic NPC assembly intermediates.

Details

show
hide
Language(s): eng - English
 Dates: 2021-05-18
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1101/2021.05.17.444137
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: bioRxiv
Source Genre: Web Page
 Creator(s):
Affiliations:
Publ. Info: -
Pages: 33 Volume / Issue: - Sequence Number: 444137 Start / End Page: - Identifier: -