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  Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide

Ng, S. C., Güttler, T., & Görlich, D. (2021). Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide. Nature Communications, 12: 4047. doi:10.1038/s41467-021-24292-5.

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Ng, S. C.1, Author           
Güttler, T.2, Author           
Görlich, D.2, Author           
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1Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society, ou_578574              
2Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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Free keywords: Intrinsically disordered proteins; Permeation and transport; Protein translocation
 Abstract: The permeability barrier of nuclear pore complexes (NPCs) controls nucleocytoplasmic transport. It retains inert macromolecules while allowing facilitated passage of importins and exportins, which in turn shuttle cargo into or out of cell nuclei. The barrier can be described as a condensed phase assembled from cohesive FG repeat domains. NPCs contain several distinct FG domains, each comprising variable repeats. Nevertheless, we now found that sequence heterogeneity is no fundamental requirement for barrier function. Instead, we succeeded in engineering a perfectly repeated 12mer GLFG peptide that self-assembles into a barrier of exquisite transport selectivity and fast transport kinetics. This barrier recapitulates RanGTPase-controlled importin- and exportin-mediated cargo transport and thus represents an ultimately simplified experimental model system. An alternative proline-free sequence forms an amyloid FG phase. Finally, we discovered that FG phases stain bright with ‘DNA-specific’ DAPI/ Hoechst probes, and that such dyes allow for a photo-induced block of nuclear transport.

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Language(s): eng - English
 Dates: 2021-06-30
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-24292-5
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Title: Nature Communications
Source Genre: Journal
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Pages: 17 Volume / Issue: 12 Sequence Number: 4047 Start / End Page: - Identifier: -