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  The total mRNA concentration buffering system in yeast is global rather than gene-specific

García-Martínez, J., Medina, D. A., Bellvís, P., Sun, M., Cramer, P., Chávez, S., et al. (2021). The total mRNA concentration buffering system in yeast is global rather than gene-specific. RNA, 27(10), 1281-1290. doi:10.1261/rna.078774.121.

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García-Martínez, J., Author
Medina, D. A., Author
Bellvís, P., Author
Sun, M.1, Author           
Cramer, P.1, Author           
Chávez, S., Author
Pérez-Ortín, J. E., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: crosstalk, transcription, mRNA decay, yeast, aneuploidy, NMD
 Abstract: Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in non sense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one.

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Language(s): eng - English
 Dates: 2021-07-162021-10
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1261/rna.078774.121
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Title: RNA
Source Genre: Journal
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Pages: - Volume / Issue: 27 (10) Sequence Number: - Start / End Page: 1281 - 1290 Identifier: -