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  Super-resolution imaging and estimation of protein copy numbers atsingle synapses with DNA-pointaccumulation for imaging innanoscale topography

Böger, C., Hafner, A.-S., Schlichthärle, T., Strauss, M. T., Malkusch, S., Endesfelder, U., et al. (2019). Super-resolution imaging and estimation of protein copy numbers atsingle synapses with DNA-pointaccumulation for imaging innanoscale topography. Neurophotonics, 6(3): 035008. doi:10.1117/1.NPh.6.3.035008.

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 Creators:
Böger, Carolin1, Author           
Hafner, Anne-Sophie2, Author           
Schlichthärle, Thomas3, 4, Author           
Strauss, Maximilian T.3, 4, Author           
Malkusch, Sebastian1, Author           
Endesfelder, U.5, Author           
Jungmann, Ralf3, 4, Author           
Schuman, Erin M.6, Author           
Heilemann, Mike1, Author           
Affiliations:
1Goethe University, Institute of Physical and Theoretical Chemistry, Frankfurt, Germany, ou_persistent22              
2Max Planck Institute for Brain Research, Max Planck Society, ou_2461692              
3Ludwigs-Maximilians Universität, Center for Nanoscience, Faculty of Physics, Munich, Germany, ou_persistent22              
4MPI of Biochemistry, Martinsried, Germany, ou_persistent22              
5Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266288              
6Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              

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Free keywords: DNA-point accumulation for imaging in nanoscale topography; molecular quantification; super-resolution imaging; synaptic proteins
 Abstract: In the brain, the strength of each individual synapse is defined by the complement of proteins present or the "local proteome." Activity-dependent changes in synaptic strength are the result of changes in this local proteome and posttranslational protein modifications. Although most synaptic proteins have been identified, we still know little about protein copy numbers in individual synapses and variations between synapses. We use DNA-point accumulation for imaging in nanoscale topography as a single-molecule super-resolution imaging technique to visualize and quantify protein copy numbers in single synapses. The imaging technique provides near-molecular spatial resolution, is unaffected by photobleaching, enables imaging of large field of views, and provides quantitative molecular information. We demonstrate these benefits by accessing copy numbers of surface AMPA-type receptors at single synapses of rat hippocampal neurons along dendritic segments.

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Language(s): eng - English
 Dates: 2019-08-21
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1117/1.NPh.6.3.035008
PMID: 31637284
PMC: PMC6795074
 Degree: -

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Title: Neurophotonics
Source Genre: Journal
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Publ. Info: Bellingham, WA, USA : SPIE
Pages: - Volume / Issue: 6 (3) Sequence Number: 035008 Start / End Page: - Identifier: ISSN: 2329-4248
CoNE: https://pure.mpg.de/cone/journals/resource/2329-4248