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  An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo

Ast, J., Novak, A. N., Podewin, T., Fine, N. H., Jones, B., Tomas, A., et al. (2021). An expanded LUXendin color palette for GLP1R detection and visualization in vitro and in vivo. ChemRxiv, 1-23. doi:10.33774/chemrxiv-2021-7rngq.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-F3B3-B Version Permalink: https://hdl.handle.net/21.11116/0000-0008-F3B4-A
Genre: Journal Article

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ChemRxiv_2021_7rngq.pdf (Preprint), 11MB
 
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 Creators:
Ast, Julia, Author
Novak, Alissa N., Author
Podewin, Tom1, Author           
Fine, Nicholas H.F., Author
Jones, Ben, Author
Tomas, Alejandra, Author
Birke, Ramona, Author
Roßmann, Kilian, Author
Mathes, Bettina1, Author           
Eichhorst, Jenny, Author
Lehmann, Martin, Author
Linnemann, Amelia K., Author
Hodson, David J., Author
Broichhagen, Johannes1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: The glucagon-like peptide-1 receptor (GLP1R) is expressed in peripheral tissues and the brain, where it exerts pleiotropic actions on metabolic and inflammatory processes. Detection and visualization of GLP1R remains challenging, partly due to a lack of validated reagents. Previously, we generated LUXendins, antagonistic red and far-red fluorescent probes for specific labeling of GLP1R in live and fixed cells/tissue. We now extend this concept to the green and near-infrared color ranges by synthesizing and testing LUXendin492, LUXendin551, LUXendin615 and LUXendin762. All four probes brightly and specifically label GLP1R in cells and pancreatic islets. Further, LUXendin551 acts as chemical beta cell reporter in preclinical rodent models, while LUXendin762 allows non-invasive imaging, highlighting differentially-accessible GLP1R populations. We thus expand the color palette of LUXendins to seven different spectra, opening up a range of experiments using widefield microscopy available in most labs through super-resolution imaging and whole animal imaging. With this, we expect that LUXendins will continue to generate novel and specific insight into GLP1R biology.

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Language(s): eng - English
 Dates: 2021-07-27
 Publication Status: Published online
 Pages: 23
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 Identifiers: URI: https://chemrxiv.org/engage/chemrxiv/article-details/60ff0d5a393cc904c94f1f18
DOI: 10.33774/chemrxiv-2021-7rngq
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Title: ChemRxiv
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 23 Identifier: -