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  A color-shifting near-infrared fluorescent aptamer-fluorophore module for live-cell RNA imaging

Zhang, J., Wang, L., Jäschke, A., & Sunbul, M. (2021). A color-shifting near-infrared fluorescent aptamer-fluorophore module for live-cell RNA imaging. Angewandte Chemie International Edition, 60(39), 21441-21448. doi:10.1002/anie.202107250.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0008-F3BD-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-0EAF-3
Genre: Journal Article

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 Creators:
Zhang, Jingye, Author
Wang, Lu1, Author           
Jäschke, Andres, Author
Sunbul, Murat, Author
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: fluorophores • RNA aptamer • RNA imaging • ratiometric imaging • SELEX
 Abstract: Fluorescent light-up RNA aptamers (FLAPs) have become promising tools for visualizing RNAs in living cells. Specific binding of FLAPs to their non-fluorescent cognate ligands results in a dramatic fluorescence increase, thereby allowing RNA imaging. Here, we present a color-shifting aptamer-fluorophore system, where the free dye is cyan fluorescent and the aptamer-dye complex is near-infrared (NIR) fluorescent. Unlike other reported FLAPs, this system enables ratiometric RNA imaging. To design the color-shifting system, we synthesized a series of environmentally sensitive benzopyrylium-coumarin hybrid fluorophores which exist in equilibrium between a cyan fluorescent spirocyclic form and a NIR fluorescent zwitterionic form. As an RNA tag, we evolved a 38-nucleotide aptamer that selectively binds the zwitterionic forms with nanomolar affinity. We used this system as a light-up RNA marker to image mRNAs in the NIR region and demonstrated its utility in ratiometric analysis of target RNAs expressed at different levels in single cells.

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Language(s): eng - English
 Dates: 2021-05-312021-07-262021-09-20
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202107250
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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 60 (39) Sequence Number: - Start / End Page: 21441 - 21448 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851