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  Regional specialization and fate specification of bone stromal cells in skeletal development

Sivaraj, K. K., Jeong, H.-W., Dharmalingam, B., Zeuschner, D., Adams, S., Potente, M., et al. (2021). Regional specialization and fate specification of bone stromal cells in skeletal development. CELL REPORTS, 36(2): 109352. doi:10.1016/j.celrep.2021.109352.

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Genre: Journal Article

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 Creators:
Sivaraj, Kishor K.1, Author              
Jeong, Hyun-Woo, Author
Dharmalingam, Backialakshmi, Author
Zeuschner, Dagmar, Author
Adams, Susanne, Author
Potente, Michael2, Author              
Adams, Ralf H., Author
Affiliations:
1Pharmacology, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591696              
2Angiogenesis & Metabolism Laboratory, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591701              

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Free keywords: VASCULAR SMOOTH-MUSCLE; MESENCHYMAL STEM-CELLS; BLOOD-VESSEL FORMATION; PROGENITOR CELLS; SINGLE-CELL; PDGFR-BETA; MARROW; IDENTIFICATION; ANGIOGENESIS; PERICYTESCell Biology;
 Abstract: Bone stroma contributes to the regulation of osteogenesis and hematopoiesis but also to fracture healing and disease processes. Mesenchymal stromal cells from bone (BMSCs) represent a heterogenous mixture of different subpopulations with distinct molecular and functional properties. The lineage relationship between BMSC subsets and their regulation by intrinsic and extrinsic factors are not well understood. Here, we show with mouse genetics, ex vivo cell differentiation assays, and transcriptional profiling that BMSCs from metaphysis (mpMSCs) and diaphysis (dpMSCs) are fundamentally distinct. Fate-tracking experiments and single-cell RNA sequencing indicate that bone-forming osteoblast lineage cells and dpMSCs, including leptin receptor-positive (LepR(+)) reticular cells in bone marrow, emerge from mpMSCs in the postnatal metaphysis. Finally, we show that BMSC fate is controlled by platelet-derived growth factor receptor beta (PDGFR beta) signaling and the transcription factor Jun-B. The sum of our findings improves our understanding of BMSC development, lineage relationships, and differentiation.

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Language(s): eng - English
 Dates: 2021-07-13
 Publication Status: Published online
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: CELL REPORTS
Source Genre: Journal
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Publ. Info: 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 36 (2) Sequence Number: 109352 Start / End Page: - Identifier: ISSN: 2211-1247