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  Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis

Bolondi, A., Haut, L., Gassaloglu, S. I., Burton, P., Kretzmer, H., Buschow, R., et al. (2021). Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis. Bio-protocol, 11(11): e4042. doi:10.21769/BioProtoc.4042.

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 Creators:
Bolondi, Adriano1, Author           
Haut, Leah1, Author
Gassaloglu, Seher Ipek2, Author           
Burton, Polly2, Author
Kretzmer, Helene1, Author           
Buschow, Rene3, Author           
Meissner, Alexander1, Author           
Herrmann, Bernhard G.2, Author           
Veenvliet, Jesse V.2, Author           
Affiliations:
1Dept. of Genome Regulation (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2379694              
2Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              
3Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              

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Free keywords: Embryoids; Gastrulation; Gastruloids; In vitro models; Morphogenesis; Organoids; Self-organization; Somites; Stem cells; Trunk-like structures
 Abstract: Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several complementary in vitro systems comprising self-organized assemblies of mouse embryonic stem cells, such as gastruloids, have been reported. We recently demonstrated that the morphogenetic potential of gastruloids can be further unlocked by the addition of a low percentage of Matrigel as an extracellular matrix surrogate. This resulted in the formation of highly organized trunk-like structures (TLSs) with a neural tube that is frequently flanked by bilateral somites. Notably, development at the molecular and morphogenetic levels is highly reminiscent of the natural embryo. To facilitate access to this powerful model, here we provide a detailed step-by-step protocol that should allow any lab with access to standard cell culture techniques to implement the culture system. This will provide the user with a means to investigate early mid-gestational mouse embryogenesis at an unprecedented spatiotemporal resolution.

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Language(s): eng - English
 Dates: 2021-06-05
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.21769/BioProtoc.4042
 Degree: -

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Title: Bio-protocol
Source Genre: Journal
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Publ. Info: Berkley : Bio Online
Pages: - Volume / Issue: 11 (11) Sequence Number: e4042 Start / End Page: - Identifier: ISSN: 2331-8325
CoNE: https://pure.mpg.de/cone/journals/resource/2331-8325