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  Temporal compartmentalization of viral infection in bacterial cells

Labarde, A., Jakutyte, L., Billaudeau, C., Fauler, B., López-Sanz, M., Ponien, P., et al. (2021). Temporal compartmentalization of viral infection in bacterial cells. Proceedings of the National Academy of Sciences of the United States of America, 118(28): e2018297118. doi:10.1073/pnas.2018297118.

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 Creators:
Labarde, Audrey , Author
Jakutyte, Lina , Author
Billaudeau, Cyrille , Author
Fauler, Beatrix1, Author              
López-Sanz, Maria, Author
Ponien, Prishila , Author
Jacquet, Eric , Author
Mielke, Thorsten1, Author              
Ayora, Silvia , Author
Carballido-López, Rut, Author
Tavares, Paulo, Author
Affiliations:
1Microscopy and Cryo-Electron Microscopy (Head: Thorsten Mielke), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              

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Free keywords: bacteriophage, virus infection, phage DNA replication, virus assembly, bacterial cell, compartmentalization
 Abstract: Virus infection causes major rearrangements in the subcellular architecture of eukaryotes, but its impact in prokaryotic cells was much less characterized. Here, we show that infection of the bacterium Bacillus subtilis by bacteriophage SPP1 leads to a hijacking of host replication proteins to assemble hybrid viral–bacterial replisomes for SPP1 genome replication. Their biosynthetic activity doubles the cell total DNA content within 15 min. Replisomes operate at several independent locations within a single viral DNA focus positioned asymmetrically in the cell. This large nucleoprotein complex is a self-contained compartment whose boundaries are delimited neither by a membrane nor by a protein cage. Later during infection, SPP1 procapsids localize at the periphery of the viral DNA compartment for genome packaging. The resulting DNA-filled capsids do not remain associated to the DNA transactions compartment. They bind to phage tails to build infectious particles that are stored in warehouse compartments spatially independent from the viral DNA. Free SPP1 structural proteins are recruited to the dynamic phage-induced compartments following an order that recapitulates the viral particle assembly pathway. These findings show that bacteriophages restructure the crowded host cytoplasm to confine at different cellular locations the sequential processes that are essential for their multiplication.

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Language(s): eng - English
 Dates: 2021-07-13
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1073/pnas.2018297118
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 118 (28) Sequence Number: e2018297118 Start / End Page: - Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230