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  En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes

Otrin, L., Witkowska, A., Marušič, N., Zhao, Z., Lira, R. d., Kyrilis, F. L., et al. (2021). En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes. Nature Communications, 12: 4972. doi:10.1038/s41467-021-25294-z.

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 Creators:
Otrin, Lado, Author
Witkowska, Agata, Author
Marušič, Nika, Author
Zhao, Ziliang1, Author              
Lira, Rafael de1, Author              
Kyrilis, Fotis L., Author
Hamdi, Farzad, Author
Ivanov, Ivan, Author
Lipowsky, Reinhard2, Author              
Kastritis, Panagiotis L., Author
Dimova, Rumiana1, Author              
Sundmacher, Kai, Author
Jahn, Reinhard, Author
Vidaković-Koch, Tanja, Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Reinhard Lipowsky, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863327              

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Free keywords: Bioinspired materials, Biomimetics, membrane structure and assembly, Nanoscale materials
 Abstract: A variety of artificial cells springs from the functionalization of liposomes with proteins. However, these models suffer from low durability without repair and replenishment mechanisms, which can be partly addressed by replacing the lipids with polymers. Yet natural membranes are also dynamically remodeled in multiple cellular processes. Here, we show that synthetic amphiphile membranes also undergo fusion, mediated by the protein machinery for synaptic secretion. We integrated fusogenic SNAREs in polymer and hybrid vesicles and observed efficient membrane and content mixing. We determined bending rigidity and pore edge tension as key parameters for fusion and described its plausible progression through cryo-EM snapshots. These findings demonstrate that dynamic membrane phenomena can be reconstituted in synthetic materials, thereby providing new tools for the assembly of synthetic protocells.

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Language(s): eng - English
 Dates: 2021-08-172021
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41467-021-25294-z
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : SpringerNature
Pages: - Volume / Issue: 12 Sequence Number: 4972 Start / End Page: - Identifier: ISSN: 2041-1723