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  Conserved and context-dependent roles for pdgfrb signaling during zebrafish vascular mural cell development

Ando, K., Shih, Y.-H., Ebarasi, L., Grosse, A., Portman, D., Chiba, A., et al. (2021). Conserved and context-dependent roles for pdgfrb signaling during zebrafish vascular mural cell development. DEVELOPMENTAL BIOLOGY, 479, 11-22. doi:10.1016/j.ydbio.2021.06.010.

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Genre: Journal Article

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 Creators:
Ando, Koji, Author
Shih, Yu-Huan, Author
Ebarasi, Lwaki, Author
Grosse, Ann, Author
Portman, Daneal, Author
Chiba, Ayano, Author
Mattonet, Kenny1, Author              
Gerri, Claudia1, Author              
Stainier, Didier Y. R.1, Author              
Mochizuki, Naoki, Author
Fukuhara, Shigetomo, Author
Betsholtz, Christer, Author
Lawson, Nathan D., Author
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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Free keywords: BASAL GANGLIA CALCIFICATION; HEPATIC STELLATE CELLS; SMOOTH-MUSCLE; ENDOTHELIAL-CELLS; RENAL GLOMERULUS; MESANGIAL CELL; PERICYTES; BETA; MUTATIONS; RECRUITMENTDevelopmental Biology; Pdgfrb; Mural cells; Pericytes; Vascular smooth muscle cells; Zebrafish;
 Abstract: Platelet derived growth factor beta and its receptor, Pdgfrb, play essential roles in the development of vascular mural cells, including pericytes and vascular smooth muscle cells. To determine if this role was conserved in zebrafish, we analyzed pdgfb and pdgfrb mutant lines. Similar to mouse, pdgfb and pdgfrb mutant zebrafish lack brain pericytes and exhibit anatomically selective loss of vascular smooth muscle coverage. Despite these defects, pdgfrb mutant zebrafish did not otherwise exhibit circulatory defects at larval stages. However, beginning at juvenile stages, we observed severe cranial hemorrhage and vessel dilation associated with loss of pericytes and vascular smooth muscle cells in pdgfrb mutants. Similar to mouse, pdgfrb mutant zebrafish also displayed structural defects in the glomerulus, but normal development of hepatic stellate cells. We also noted defective mural cell investment on coronary vessels with concomitant defects in their development. Together, our studies support a conserved requirement for Pdgfrb signaling in mural cells. In addition, these zebrafish mutants provide an important model for definitive investigation of mural cells during early embryonic stages without confounding secondary effects from circulatory defects.

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Language(s): eng - English
 Dates: 2021-07-242021-11
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: DEVELOPMENTAL BIOLOGY
Source Genre: Journal
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Publ. Info: 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA : ACADEMIC PRESS INC ELSEVIER SCIENCE
Pages: - Volume / Issue: 479 Sequence Number: - Start / End Page: 11 - 22 Identifier: ISSN: 0012-1606