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  Transport Proteins on Solid-Supported Membranes: From Basic Research to Drug Discovery

Fendler, K., Klingenberg, M., Leblanc, G., DePont, J. J. H., Kelety, B., Dörner, W., et al. (2004). Transport Proteins on Solid-Supported Membranes: From Basic Research to Drug Discovery. In V. M. Vladimir M. (Ed.), Springer Series on Chemical Sensors and Biosensors (Methods and Applications) (pp. 331-349). Berlin, Heidelberg: Springer-Verlag.

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 Creators:
Fendler, Klaus1, Author              
Klingenberg, Martin2, Author
Leblanc, Gerard2, Author
DePont, Jan Joep H.H.M.2, Author
Kelety, Béla1, Author              
Dörner, Wolfgang2, Author
Bamberg, Ernst1, Author              
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2External Organizations, ou_persistent22              

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Free keywords: Solid-supported membrane; Electrogenic; Membrane protein; Drug discovery; Drug screening
 Abstract: Solid-supported membranes (SSM) can be used as capacitive electrodes for the investigation of electrogenic transport proteins (ion pumps). Membrane fragments or liposomes that contain the transport protein are adsorbed on the SSM. The proteins are activated by supplying a substrate via a rapid solution exchange at the SSM. The charge translocations during the reaction cycle of the transport protein can be measured via the capacitance of the SSM. The SSM and the adsorbed membrane fragments or proteoliposomes represent a rugged structure that can be used in basic research and drug discovery. On the basis of a few examples we demonstrate how this technique can be applied to the investigation of the transport mechanism of membrane proteins. In addition, we present the application of the system in drug discovery. A sensor based on the SSM technology represents a promising system for the rapid screening of pharmaceutically relevant compounds for transport proteins.

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Language(s): eng - English
 Dates: 2004
 Publication Status: Published in print
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-662-05204-4_13
 Degree: -

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Title: Springer Series on Chemical Sensors and Biosensors (Methods and Applications)
  Subtitle : Ultrathin Electrochemical Chemo- and Biosensors
  Subtitle : Technology and Performance
Source Genre: Series
 Creator(s):
Vladimir M., Vladimir M.1, Editor
Affiliations:
1 Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg, Germany, ou_persistent22            
Publ. Info: Berlin, Heidelberg : Springer-Verlag
Pages: 358 Volume / Issue: 2 Sequence Number: - Start / End Page: 331 - 349 Identifier: DOI: 10.1007/978-3-662-05204-4
ISBN: 978-3-540-21285-0
ISBN: 978-3-662-05204-4
ISSN: 1612-7617