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  The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions

Hänsel, R., Löhr, F., Foldynová-Trantírková, S., Bamberg, E., Trantírek, L., & Dötsch, V. (2011). The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions. Nucleic Acids Research, 39(13), 5768-5775. doi:10.1093/nar/gkr174.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-2CD3-8 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-2CD4-7
Genre: Journal Article

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 Creators:
Hänsel, Robert1, Author
Löhr, Frank1, Author
Foldynová-Trantírková, Silvie2, Author
Bamberg, Ernst3, Author           
Trantírek, Lukás4, Author
Dötsch, Volker1, Author
Affiliations:
1Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University, 60438 Frankfurt am Main, Germany, ou_persistent22              
2Biology Centre, v.v.i., AS CR, Branisovska 31, 37005 Ceske Budejovice, Czech Republic, ou_persistent22              
3Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
4Department of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands, ou_persistent22              

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 Abstract: G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic.

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Language(s): eng - English
 Dates: 2011-03-092010-12-172011-03-102011-03-202011-07-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/nar/gkr174
PMID: 21450807
PMC: PMC3141269
 Degree: -

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Title: Nucleic Acids Research
  Other : Nucleic Acids Res.
Source Genre: Journal
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Pages: - Volume / Issue: 39 (13) Sequence Number: - Start / End Page: 5768 - 5775 Identifier: ISSN: 0301-5610
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000262810