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  Functional and Structural Properties of Highly Responsive Somatosensory Neurons in Mouse Barrel Cortex

Barz, C. S., Garderes, P. M., Ganea, D. A., Reischauer, S., Feldmeyer, D., & Haiss, F. (2021). Functional and Structural Properties of Highly Responsive Somatosensory Neurons in Mouse Barrel Cortex. CEREBRAL CORTEX, 31(10), 4533-4553. doi:10.1093/cercor/bhab104.

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 Creators:
Barz, C. S., Author
Garderes, P. M., Author
Ganea, D. A., Author
Reischauer, S.1, Author              
Feldmeyer, D., Author
Haiss, F., Author
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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Free keywords: PYRAMIDAL CELLS; LAYER 2/3; SYNAPTIC CONNECTIONS; RECEPTIVE-FIELDS; CORTICAL-NEURONS; SPARSE; REPRESENTATION; MECHANISMS; REORGANIZATION; COMMUNICATIONNeurosciences & Neurology; barrel cortex; patch clamp; somatosensory; sparse coding; two-photon imaging;
 Abstract: Sparse population activity is a well-known feature of supragranular sensory neurons in neocortex. The mechanisms underlying sparseness are not well understood because a direct link between the neurons activated in vivo, and their cellular properties investigated in vitro has been missing. We used two-photon calcium imaging to identify a subset of neurons in layer L2/3 (L2/3) of mouse primary somatosensory cortex that are highly active following principal whisker vibrotactile stimulation. These high responders (HRs) were then tagged using photoconvertible green fluorescent protein for subsequent targeting in the brain slice using intracellular patch-clamp recordings and biocytin staining. This approach allowed us to investigate the structural and functional properties of HRs that distinguish them from less active control cells. Compared to less responsive L2/3 neurons, HRs displayed increased levels of stimulus-evoked and spontaneous activity, elevated noise and spontaneous pairwise correlations, and stronger coupling to the population response. Intrinsic excitability was reduced in HRs, while we found no evidence for differences in other electrophysiological and morphological parameters. Thus, the choice of which neurons participate in stimulus encoding may be determined largely by network connectivity rather than by cellular structure and function.

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Language(s): eng - English
 Dates: 2021-05-082021-10
 Publication Status: Published in print
 Pages: 21
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000695807700010
DOI: 10.1093/cercor/bhab104
PMID: 33963394
 Degree: -

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Title: CEREBRAL CORTEX
Source Genre: Journal
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Publ. Info: JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA : OXFORD UNIV PRESS INC
Pages: - Volume / Issue: 31 (10) Sequence Number: - Start / End Page: 4533 - 4553 Identifier: ISSN: 1047-3211