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  Exploring Translational Control of Maternal mRNAs in Zebrafish

Winata, C. L., Lapinski, M., Ismail, H., Mathavan, S., & Sampath, P. (2021). Exploring Translational Control of Maternal mRNAs in Zebrafish. In GERMLINE DEVELOPMENT IN THE ZEBRAFISH: Methods and Protocols (pp. 367-380). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA: HUMANA PRESS INC. doi:10.1007/978-1-0716-0970-5_29.

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 Creators:
Winata, Cecilia Lanny1, Author              
Lapinski, Maciej, Author
Ismail, Hisyam, Author
Mathavan, Sinnakaruppan, Author
Sampath, Prabha, Author
Affiliations:
1Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591695              

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Free keywords: POLY(A) POLYMERASE; LENGTH; DECAYBiochemistry & Molecular Biology; Marine & Freshwater Biology; Maternal RNA; Translational regulation; Polysome profiling; Cytoplasmic polyadenylation; Zebrafish;
 Abstract: The study of translational regulation requires reliable measurement of both mRNA levels and protein synthesis. Cytoplasmic polyadenylation is a prevalent mode of translational regulation during oogenesis and early embryogenesis. Here the length of the poly(A) tail of an mRNA is coupled to its translatability. We describe a protocol to identify translationally regulated genes and measure their translation rate in the early zebrafish embryo using genome-wide polysome profiling. This protocol relies on the isolation of mRNA by means of an rRNA depletion strategy, which avoids capture bias due to short poly(A) tail that can occur when using conventional oligo(dT)-based methods. We also present a simple PCR-based method to measure the poly(A) tail length of selected mRNAs.

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Language(s): eng - English
 Dates: 2021-02-202021
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: GERMLINE DEVELOPMENT IN THE ZEBRAFISH: Methods and Protocols
  Alternative Title : Methods Mol. Biol.
  Alternative Title : METHODS MOL BIOL
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : HUMANA PRESS INC
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 367 - 380 Identifier: ISSN: 1064-3745
ISBN: 978-1-0716-0970-5; 978-1-0716-0969-9

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Alternative Title : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: -
Pages: - Volume / Issue: 2218 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745