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  Multiplexed bio-imaging using cadmium telluride quantum dots synthesized by mathematically derived process parameters in a continuous flow active microreactor

Pandey, S., Mukherjee, D., Kshirsagar, P., Patra, C., & Bodas, D. (2021). Multiplexed bio-imaging using cadmium telluride quantum dots synthesized by mathematically derived process parameters in a continuous flow active microreactor. MATERIALS TODAY BIO, 11: 100123. doi:10.1016/j.mtbio.2021.100123.

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Pandey, S., Author
Mukherjee, D.1, Author              
Kshirsagar, P., Author
Patra, C., Author
Bodas, D., Author
Affiliations:
1IMPRS, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_3242057              

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Free keywords: AQUEOUS SYNTHESIS; NANOPARTICLES; CDSE; CDTEEngineering; Materials Science; Fluorescent nanocrystals; Multiplexed imaging; PDMS; Dimensional analysis; Microreactor synthesis;
 Abstract: Quantum dots (QDs) are semiconductor nanocrystals with unique size-tunable emissions. To obtain a precise emission spectrum, monodispersity in size is imperative, which is achieved by controlling the reaction kinetics in a continuous flow of active microreactors. Further, a multivariate approach (dimensional analysis) is employed to impose stringent control on the reaction process resulting in monodispersed preparation of cadmium telluride (CdTe) quantum dots. Dimensional analysis knits multiple variables into a dimensionless mathematical form which not only predicts parameters precisely to obtain narrow size tunability but also guarantees reproducibility in synthesis. Analytical, structural, and optical characterization of the microreactor synthesized poly-dimethylsiloxane (PDMS) coated CdTe QDs reveal quantum efficient (61.5%), photostable (44%), and biocom-patible nanocrystals of 5-15 nm. Further, PDMS-coated QDs (P-QDs) are conjugated with organelle-specific antibodies/biomarkers for in-vitro imaging in NIH 3T3 cells. Likewise, proliferating cell nuclear antigen (PCNA) and anti-myosin (MF20), cardiomyocytes antibodies are conjugated with P-QDs (red and green, respec-tively) to image the zebrafish's cardiac tissue. Antibodies tagged with quantum dots are imaged simultaneously using confocal microscopy. Thus, multiplexed bio-imaging of in-vitro and zebrafish tissue is demonstrated suc-cessfully. The results indicate the suitability of continuous flow active microreactor in conjunction with the mathematical prediction of process parameters to synthesize reproducibly monodispersed and quantum efficient QDs.

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Language(s): eng - English
 Dates: 2021-07-24
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: MATERIALS TODAY BIO
Source Genre: Journal
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Publ. Info: RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS : ELSEVIER
Pages: - Volume / Issue: 11 Sequence Number: 100123 Start / End Page: - Identifier: ISSN: 2590-0064