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  A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis

Anchimiuk, A., Lioy, V. S., Bock, F. P., Minnen, A., Boccard, F., & Gruber, S. (2021). A low Smc flux avoids collisions and facilitates chromosome organization in Bacillus subtilis. eLife, 10: e65467. doi:10.7554/eLife.65467.

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 Creators:
Anchimiuk, Anna1, Author
Lioy, Virginia S.1, Author
Bock, Florian Patrick1, Author
Minnen, Anita2, Author              
Boccard, Frederic1, Author
Gruber, Stephan1, Author
Affiliations:
1external, ou_persistent22              
2Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              

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Free keywords: PROTEIN SPO0J PARB; COLI CHROMOSOME; CONDENSIN; ORIGIN; REPLICATION; DNA; SEGREGATION; PROMOTES; ARMS; ARCHITECTURELife Sciences & Biomedicine - Other Topics;
 Abstract: SMC complexes are widely conserved ATP-powered DNA-loop-extrusion motors indispensable for organizing and faithfully segregating chromosomes. How SMC complexes translocate along DNA for loop extrusion and what happens when two complexes meet on the same DNA molecule is largely unknown. Revealing the origins and the consequences of SMC encounters is crucial for understanding the folding process not only of bacterial, but also of eukaryotic chromosomes. Here, we uncover several factors that influence bacterial chromosome organization by modulating the probability of such clashes. These factors include the number, the strength, and the distribution of Smc loading sites, the residency time on the chromosome, the translocation rate, and the cellular abundance of Smc complexes. By studying various mutants, we show that these parameters are fine-tuned to reduce the frequency of encounters between Smc complexes, presumably as a risk mitigation strategy. Mild perturbations hamper chromosome organization by causing Smc collisions, implying that the cellular capacity to resolve them is limited. Altogether, we identify mechanisms that help to avoid Smc collisions and their resolution by Smc traversal or other potentially risky molecular transactions.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000685206600001
DOI: 10.7554/eLife.65467
 Degree: -

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Title: eLife
Source Genre: Journal
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Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 10 Sequence Number: e65467 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X