English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Binding of Rap1 and Riam to Talin1 Fine-Tune beta 2 Integrin Activity During Leukocyte Trafficking

Bromberger, T., Klapproth, S., Rohwedder, I., Weber, J., Pick, R., Mittmann, L., et al. (2021). Binding of Rap1 and Riam to Talin1 Fine-Tune beta 2 Integrin Activity During Leukocyte Trafficking. Frontiers in Immunology, 12: 702345. doi:10.3389/fimmu.2021.702345.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Bromberger, Thomas1, Author              
Klapproth, Sarah2, Author
Rohwedder, Ina2, Author
Weber, Jasmin2, Author
Pick, Robert2, Author
Mittmann, Laura2, Author
Min-Weißenhorn, Soo Jin3, Author              
Reichel, Christoph A.2, Author
Scheiermann, Christoph2, Author
Sperandio, Markus2, Author
Moser, Markus1, Author              
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
2external, ou_persistent22              
3Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

Content

show
hide
Free keywords: SUBCELLULAR-LOCALIZATION; EFFECTOR RIAM; ACTIVATION; DOMAIN; KINDLINS; SWITCH; LFA-1Immunology; talin; Riam; Rap1; leukocyte adhesion; leukocyte rolling; integrin activation; leukocyte trafficking;
 Abstract: beta 2 integrins mediate key processes during leukocyte trafficking. Upon leukocyte activation, the structurally bent beta 2 integrins change their conformation towards an extended, intermediate and eventually high affinity conformation, which mediate slow leukocyte rolling and firm arrest, respectively. Translocation of talin1 to integrin adhesion sites by interactions with the small GTPase Rap1 and the Rap1 effector Riam precede these processes. Using Rap1 binding mutant talin1 and Riam deficient mice we show a strong Riam-dependent T cell homing process to lymph nodes in adoptive transfer experiments and by intravital microscopy. Moreover, neutrophils from compound mutant mice exhibit strongly increased rolling velocities to inflamed cremaster muscle venules compared to single mutants. Using Hoxb8 cell derived neutrophils generated from the mutant mouse strains, we show that both pathways regulate leukocyte rolling and adhesion synergistically by inducing conformational changes of the beta 2 integrin ectodomain. Importantly, a simultaneous loss of both pathways results in a rolling phenotype similar to talin1 deficient neutrophils suggesting that beta 2 integrin regulation primarily occurs via these two pathways.

Details

show
hide
Language(s): eng - English
 Dates: 2021
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000693546800001
DOI: 10.3389/fimmu.2021.702345
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Immunology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND : FRONTIERS MEDIA SA
Pages: - Volume / Issue: 12 Sequence Number: 702345 Start / End Page: - Identifier: ISSN: 1664-3224