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  Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation

Hoefig, K. P., Reim, A., Gallus, C., Wong, E. H., Behrens, G., Conrad, C., et al. (2021). Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. Nature Communications, 12(1): 5208. doi:10.1038/s41467-021-25345-5.

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 Creators:
Hoefig, Kai P.1, Author
Reim, Alexander2, Author              
Gallus, Christian1, Author
Wong, Elaine H.1, Author
Behrens, Gesine1, Author
Conrad, Christine1, Author
Xu, Meng1, Author
Kifinger, Lisa1, Author
Ito-Kureha, Taku1, Author
Defourny, Kyra A. Y.1, Author
Geerlof, Arie1, Author
Mautner, Josef1, Author
Hauck, Stefanie M.1, Author
Baumjohann, Dirk1, Author
Feederle, Regina1, Author
Mann, Matthias2, Author              
Wierer, Michael2, Author              
Glasmacher, Elke1, Author
Heissmeyer, Vigo1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: INDUCIBLE COSTIMULATOR; BINDING PROTEINS; STEM-LOOP; ICOS; DIFFERENTIATION; EXPRESSION; PROTEOME; EVENTS; IDENTIFICATION; DEGRADATIONScience & Technology - Other Topics;
 Abstract: Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costi-mulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select similar to 50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 (1) Sequence Number: 5208 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723