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  The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity

Sun, C., Nold, A., Fusco, C., Rangaraju, V., Tchumatchenko, T., Heilemann, M., et al. (2021). The prevalence and specificity of local protein synthesis during neuronal synaptic plasticity. Sci. Adv., 7(38): eabj0790. doi:10.1126/sciadv.abj0790.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-48CB-2 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-8E1E-7
Genre: Journal Article

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2021
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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC)
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https://creativecommons.org/licenses/by-nc/4.0/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448450/ (Any fulltext)
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 Creators:
Sun , Chao1, Author
Nold, Andreas2, 3, Author
Fusco, Claudia1, Author
Rangaraju, Vidhya1, Author
Tchumatchenko, Tatjana2, 3, Author           
Heilemann, Mike4, Author           
Schuman, Erin M.1, Author           
Affiliations:
1Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society, ou_2461710              
2Theory of neural dynamics Group, Max Planck Institute for Brain Research, Max Planck Society, ou_2461711              
3Institute of Experimental Epileptology and Cognition Research, Life and Brain Center, Universitätsklinikum Bonn, Venusberg-Campus 1, 53127 Bonn, Germany., ou_persistent22              
4Goethe University, Institute of Physics and Theoretical Chemistry, Frankfurt, Germany, ou_persistent22              

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 Abstract: To supply proteins to their vast volume, neurons localize mRNAs and ribosomes in dendrites and axons. While local protein synthesis is required for synaptic plasticity, the abundance and distribution of ribosomes and nascent proteins near synapses remain elusive. Here, we quantified the occurrence of local translation and visualized the range of synapses supplied by nascent proteins during basal and plastic conditions. We detected dendritic ribosomes and nascent proteins at single-molecule resolution using DNA-PAINT and metabolic labeling. Both ribosomes and nascent proteins positively correlated with synapse density. Ribosomes were detected at ~85% of synapses with ~2 translational sites per synapse; ~50% of the nascent protein was detected near synapses. The amount of locally synthesized protein detected at a synapse correlated with its spontaneous Ca2+ activity. A multifold increase in synaptic nascent protein was evident following both local and global plasticity at respective scales, albeit with substantial heterogeneity between neighboring synapses.

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Language(s): eng - English
 Dates: 2021-04-192021-07-272021-09-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1126/sciadv.abj0790
PMID: 34533986
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Project name : EMBO Fellowship
Grant ID : EMBO ALTF 860-2018
Funding program : EMBO long-term postdoctoral fellowship
Funding organization : EMBO
Project name : HFSP Fellowship
Grant ID : LT000737/2019-C
Funding program : HFSP Cross-Disciplinary Fellowship
Funding organization : HFSP
Project name : Joachim Herz Stiftung Add-on Fellowship for Interdisciplinary Life Science
Grant ID : -
Funding program : -
Funding organization : -
Project name : DFG CRC1080: Molecular and Cellular Mechanisms of Neural Homeostasis
Grant ID : 221828878
Funding program : Collaborative Research Centres
Funding organization : Deutsche Forschungsgemeinschaft (DFG)
Project name : ERC-AdG NeuroRibo
Grant ID : 743216
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : SFB 902: Molecular Principles of RNA-based Regulation
Grant ID : 161793742
Funding program : Collaborative Research Centres
Funding organization : Deutsche Forschungsgemeinschaft (DFG)

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Title: Sci. Adv.
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 7 (38) Sequence Number: eabj0790 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548