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  Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser

E, J., Stransky, M., Jurek, Z., Fortmann-Grote, C., Juha, L., Santra, R., et al. (2021). Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser. Scientific Reports, 11: 17976. doi:10.1038/s41598-021-97142-5.

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 Urheber:
E, Juncheng, Autor
Stransky, Michal, Autor
Jurek, Zoltan, Autor
Fortmann-Grote, Carsten1, Autor           
Juha, Libor, Autor
Santra, Robin, Autor
Ziaja, Beata, Autor
Mancuso, Adrian P., Autor
Affiliations:
1Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              

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 Zusammenfassung: We present a computational case study of X-ray single-particle imaging of hydrated proteins on an example of 2-Nitrogenase–Iron protein covered with water layers of various thickness, using a start-to-end simulation platform and experimental parameters of the SPB/SFX instrument at the European X-ray Free-Electron Laser facility. The simulations identify an optimal thickness of the water layer at which the effective resolution for imaging the hydrated sample becomes significantly higher than for the non-hydrated sample. This effect is lost when the water layer becomes too thick. Even though the detailed results presented pertain to the specific sample studied, the trends which we identify should also hold in a general case. We expect these findings will guide future single-particle imaging experiments using hydrated proteins.

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Sprache(n): eng - English
 Datum: 2021-05-072021-08-192021-09-092021
 Publikationsstatus: Erschienen
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 Identifikatoren: DOI: 10.1038/s41598-021-97142-5
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Titel: Scientific Reports
  Kurztitel : Sci. Rep.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London, UK : Nature Publishing Group
Seiten: - Band / Heft: 11 Artikelnummer: 17976 Start- / Endseite: - Identifikator: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322