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  Brassinosteroids influence arabidopsis hypocotyl graviresponses through changes in mannans and cellulose

Somssich, M., Vandenbussche, F., Ivakov, A., Funke, N., Ruprecht, C., Vissenberg, K., et al. (2021). Brassinosteroids influence arabidopsis hypocotyl graviresponses through changes in mannans and cellulose. Plant and Cell Physiology, 62(4), 678-692. doi:10.1093/pcp/pcab024.

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 Creators:
Somssich, Marc, Author
Vandenbussche, Filip, Author
Ivakov, Alexander, Author
Funke, Norma, Author
Ruprecht, Colin1, Author              
Vissenberg, Kris, Author
VanDer Straeten, Dominique, Author
Persson, Staffan, Author
Suslov, Dmitry, Author
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Arabidopsis thaliana; Brassinosteroids; Cell wall; Cellulose; Gravitropism; Mannan
 Abstract: The force of gravity is a constant environmental factor. Plant shoots respond to gravity through negative gravitropism and gravity resistance. These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground. We took advantage of the effect of brassinosteroids (BRs) on the two types of graviresponses in Arabidopsis thaliana hypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth. The ability of etiolated Arabidopsis seedlings to grow upward was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of BR biosynthesis. These effects were accompanied by changes in cell wall mechanics and composition. Cell wall biochemical analyses, confocal microscopy of the cellulose-specific pontamine S4B dye and cellular growth analyses revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre organization, cell expansion at the hypocotyl base and mannan content. Indeed, a longitudinal reorientation of cellulose fibres and growth inhibition at the base of hypocotyls supported their upright posture whereas the presence of mannans reduced gravitropic bending. The negative effect of mannans on gravitropism is a new function for this class of hemicelluloses. We also found that EBL interferes with upright growth of hypocotyls through their uneven thickening at the base.

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Language(s): eng - English
 Dates: 2021-02-112021
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1093/pcp/pcab024
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Title: Plant and Cell Physiology
  Other : Plant Cell Physiol.
Source Genre: Journal
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Publ. Info: Kyoto [etc.] : Japanese Society of Plant Physiologists.
Pages: - Volume / Issue: 62 (4) Sequence Number: - Start / End Page: 678 - 692 Identifier: ISSN: 0032-0781