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  Crystallization and preliminary X-ray diffraction studies of methyl-coenzyme M reductase from methanobacterium thermoautotrophicum

Shima, S., Goubeaud, M., Vinzenz, D., Thauer, R. K., & Ermler, U. (1997). Crystallization and preliminary X-ray diffraction studies of methyl-coenzyme M reductase from methanobacterium thermoautotrophicum. Journal of Biochemistry, 121(5), 829-830. doi:10.1093/oxfordjournals.jbchem.a021660.

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 Creators:
Shima, Seigo1, Author           
Goubeaud, Marcel 2, Author
Vinzenz, Daniela3, Author           
Thauer, Rudolf Kurt2, Author           
Ermler, Ulrich3, Author           
Affiliations:
1Department-Independent Research Group Microbial Protein Structure, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266277              
2Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              
3Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: coenzyme F430, methanogenic Archaea, methyl-coenzyme M reductase, protein crystallization, X-ray crystallography
 Abstract: Methyl-coenzyme M reductase isoenzyme I from the methanogenic Archaeon, Methanobacterium thermoautotrophicum (strain Marburg), was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as the precipitant displayed space group P2(1), with unit cell parameters of a=83.2 A, b=117.4 A, c=125.1 A, and beta= 92.6 degrees, and diffracted at better than 2.8 A resolution. Crystal form P grown from polyethylene glycol 400 belonged to space group P21, and had unit cell parameters of a=83.1 A, b=120.2 A, c=123.1 A, and beta=91.7 degrees, diffracting at least to 1.7 A resolution. Both crystal forms have one molecule per asymmetric unit and are suitable for X-ray structure analysis.

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Language(s): eng - English
 Dates: 1997-02-241997-05-01
 Publication Status: Issued
 Pages: 2
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Tokyo, Japan : Japanese Biochemical Society
Pages: - Volume / Issue: 121 (5) Sequence Number: - Start / End Page: 829 - 830 Identifier: ISSN: 0021-924X
CoNE: https://pure.mpg.de/cone/journals/resource/954925246071