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  Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes

Malik, V. A., Di Benedetto, B., & Jakovcevski, M. (2019). Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes. In ASTROCYTES: METHODS AND PROTOCOLS (pp. 219-229).

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-7726-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-7727-6
Genre: Conference Paper

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 Creators:
Malik, Victoria Adelheid, Author
Di Benedetto, Barbara, Author
Jakovcevski, Mira1, Author           
Affiliations:
1Dept. Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Max Planck Society, ou_2035294              

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 Abstract: Chromatin immunoprecipitation (ChIP) in conjunction with qPCR or next generation sequencing (ChIPseq) is used to detect protein-DNA interaction. Typically, DNA bound to a protein of interest is captured with an antibody against this protein, and DNA is then purified from DNA-protein complexes. Here, we describe a native Chromatin immunoprecipitation (N-ChIP) approach which is an efficient ChIP method with high resolution for histone modifications and a number of transcription factors. This protocol has been tailored for cultured primary rat astrocytes, and we included the preparation of astrocytic cell cultures in this protocol.

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 Dates: 2019
 Publication Status: Issued
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 Identifiers: ISI: 000620741300014
DOI: 10.1007/978-1-4939-9068-9_15
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Title: ASTROCYTES: METHODS AND PROTOCOLS
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 219 - 229 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-9068-9; 978-1-4939-9067-2

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Title: Methods in Molecular Biology
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Pages: - Volume / Issue: 1938 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745