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  Visualization of the mechanosensitive ion channel MscS under membrane tension

Zhang, Y., Daday, C., Gu, R. X., Cox, C. D., Martinac, B., de Groot, B. L., et al. (2021). Visualization of the mechanosensitive ion channel MscS under membrane tension. Nature, 590(7846), 509-514. doi:10.1038/s41586-021-03196-w.

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Zhang, Y., Author
Daday, C.1, Author              
Gu, R. X.2, Author              
Cox, C. D., Author
Martinac, B., Author
de Groot, B. L.2, Author              
Walz, T., Author
Affiliations:
1Research Group of Computational Biomolecular Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_578573              
2Research Group of Computational Biomolecular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578573              

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Free keywords: Cryoelectron microscopy; Membrane biophysics
 Abstract: Mechanosensitive channels sense mechanical forces in cell membranes and underlie many biological sensing processes. However, how exactly they sense mechanical force remains under investigation. The bacterial mechanosensitive channel of small conductance, MscS, is one of the most extensively studied mechanosensitive channels, but how it is regulated by membrane tension remains unclear, even though the structures are known for its open and closed states. Here we used cryo-electron microscopy to determine the structure of MscS in different membrane environments, including one that mimics a membrane under tension. We present the structures of MscS in the subconducting and desensitized states, and demonstrate that the conformation of MscS in a lipid bilayer in the open state is dynamic. Several associated lipids have distinct roles in MscS mechanosensation. Pore lipids are necessary to prevent ion conduction in the closed state. Gatekeeper lipids stabilize the closed conformation and dissociate with membrane tension, allowing the channel to open. Pocket lipids in a solvent-exposed pocket between subunits are pulled out under sustained tension, allowing the channel to transition to the subconducting state and then to the desensitized state. Our results provide a mechanistic underpinning and expand on the ‘force-from-lipids’ model for MscS mechanosensation.

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Language(s): eng - English
 Dates: 2021-02-102021-02-18
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41586-021-03196-w
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 590 (7846) Sequence Number: - Start / End Page: 509 - 514 Identifier: -