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  Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves

Juranek, J. K., Mukherjee, K., Jahn, R., & Li, J.-Y. (2021). Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves. Biochemical and Biophysical Research Communications, 559, 92-98. doi:10.1016/j.bbrc.2021.04.041.

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Juranek, J. K., Author
Mukherjee, K., Author
Jahn, R.1, Author              
Li, J.-Y., Author
Affiliations:
1Laboratory of Neurobiology, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_3049887              

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Free keywords: Active zone; Synaptic vesicle; Presynapse; Motor neuron; Sciatic nerve
 Abstract: Synaptic transmission is mediated by neurotransmitters that are stored in synaptic vesicles (SV) and released at the synaptic active zone (AZ). While in recent years major progress has been made in unraveling the molecular machinery responsible for SV docking, fusion and exocytosis, the mechanisms governing AZ protein and SV trafficking through axons still remain unclear. Here, we performed stop-flow nerve ligation to examine axonal trafficking of endogenous AZ and SV proteins. Rat sciatic nerves were collected 1 h, 3 h and 8 h post ligation and processed for immunohistochemistry and electron microscopy. First, we followed the transport of an integral synaptic vesicle protein, SV2A and a SV-associated protein involved in SV trafficking, Rab3a, and observed that while SV2A accumulated on both sides of ligation, Rab3a was only noticeable in the proximal segment of the ligated nerve indicating that only SV trans-membrane protein SV2A displayed a bi-directional axonal transport. We then demonstrate that multiple AZ proteins accumulate rapidly on either side of the ligation with a timescale similar to that of SV2A. Overall, our data uncovers an unexpected robust bi-directional, coordinated -trafficking of SV and AZ proteins in peripheral nerves. This implies that pathological disruption of axonal trafficking will not only impair trafficking of newly synthesized proteins to the synapse but will also affect retrograde transport, leading to neuronal dysfunction and likely neurodegeneration.

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Language(s): eng - English
 Dates: 2021-04-292021-06-25
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bbrc.2021.04.041
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Title: Biochemical and Biophysical Research Communications
Source Genre: Journal
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Pages: - Volume / Issue: 559 Sequence Number: - Start / End Page: 92 - 98 Identifier: -