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  Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture

Bashirzadeh, Y., Redford, S. A., Lorpaiboon, C., Groaz, A., Litschel, T., Schwille, P., et al. (2021). Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture. Communications Biology, 4(1): 1136. doi:10.1038/s42003-021-02653-6.

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 Creators:
Bashirzadeh, Yashar1, Author
Redford, Steven A.1, Author
Lorpaiboon, Chatipat1, Author
Groaz, Alessandro1, Author
Litschel, Thomas2, Author           
Schwille, Petra2, Author           
Hocky, Glen M.1, Author
Dinner, Aaron R.1, Author
Liu, Allen P.1, Author
Moghimianavval, Hossein1, Author
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: ALPHA-ACTININ; IN-VITRO; PERSISTENCE LENGTH; MECHANICS; DYNAMICS; FASCIN; ORGANIZATION; FILOPODIA; BUNDLES; RECONSTITUTIONLife Sciences & Biomedicine - Other Topics; Science & Technology - Other Topics;
 Abstract: The proteins that make up the actin cytoskeleton can self-assemble into a variety of structures. In vitro experiments and coarse-grained simulations have shown that the actin crosslinking proteins alpha-actinin and fascin segregate into distinct domains in single actin bundles with a molecular size-dependent competition-based mechanism. Here, by encapsulating actin, alpha-actinin, and fascin in giant unilamellar vesicles (GUVs), we show that physical confinement can cause these proteins to form much more complex structures, including rings and asters at GUV peripheries and centers; the prevalence of different structures depends on GUV size. Strikingly, we found that alpha-actinin and fascin self-sort into separate domains in the aster structures with actin bundles whose apparent stiffness depends on the ratio of the relative concentrations of alpha-actinin and fascin. The observed boundary-imposed effect on protein sorting may be a general mechanism for creating emergent structures in biopolymer networks with multiple crosslinkers.
By encapsulating proteins in giant unilamellar vesicles, Bashirzadeh et al find that actin crosslinkers, alpha-actinin and fascin, can self-assemble with actin into complex structures that depend on the degree of confinement. Further analysis and modeling show that alpha-actinin and fascin sort to separate domains of these structures. These insights may be generalizable to other biopolymer networks containing crosslinkers.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Communications Biology
Source Genre: Journal
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Publ. Info: London : Springer Nature
Pages: - Volume / Issue: 4 (1) Sequence Number: 1136 Start / End Page: - Identifier: ISSN: 2399-3642
CoNE: https://pure.mpg.de/cone/journals/resource/2399-3642