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  Agrobacterium-Mediated Seedling Transformation to Measure Circadian Rhythms in Arabidopsis

Ting, M. K. Y., Zoschke, R., & Haydon, M. J. (2021). Agrobacterium-Mediated Seedling Transformation to Measure Circadian Rhythms in Arabidopsis. In D. Staiger, & S. Davis (Eds.), Plant Circadian Networks.

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 Creators:
Ting, M. K. Y.1, Author              
Zoschke, R.1, Author              
Haydon, Michael J.2, Author
Staiger, Dorothee2, Contributor
Davis, Seth2, Contributor
Davis, Amanda Melaragno2, Contributor
Affiliations:
1Translational Regulation in Plants, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2324691              
2external, ou_persistent22              

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 Abstract: Circadian clocks are endogenous timing mechanisms that allow an organism to adapt cellular processes in anticipation of predictable changes in the environment. Luciferase reporters are well utilized as an effective, nondestructive method to measure circadian rhythms of promoter activity in Arabidopsis. Obtaining stable transgenic reporter lines can be laborious. Here, we report a protocol for Agrobacterium-mediated seedling transformation tailored for plant circadian studies. We show that period estimates generated from wild-type and clock-mutant seedlings transformed with circadian luciferase reporters are similar to rhythms obtained from equivalent stable transgenic seedlings. These experiments demonstrate the versatility and robustness of the protocol for testing new constructs or quickly assessing circadian effects in any genotype of interest.

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Language(s): eng - English
 Dates: 2021-10-22
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-1912-4_5
 Degree: -

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Title: Plant Circadian Networks
Source Genre: Book
 Creator(s):
Staiger, D., Editor
Davis, S., Editor
Davis, A.M., Author
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -

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Title: Methods in Molecular Biology
Source Genre: Series
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Publ. Info: New York, NY : Humana, 2.
Pages: - Volume / Issue: 2398 Sequence Number: - Start / End Page: 57 - 64 Identifier: ISBN: 978-1-0716-1911-7