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  The Salmonella type III secretion system inner rod protein PrgJ is partially folded

Zhong, D., Lefebre, M., Kaur, K., McDowell, M. A., Gdowski, C., Jo, S., et al. (2012). The Salmonella type III secretion system inner rod protein PrgJ is partially folded. The Journal of Biological Chemistry, 287(30), 25303-25311. doi:10.1074/jbc.M112.381574.

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 Creators:
Zhong, Dalian1, Author
Lefebre, Matthew1, Author
Kaur, Kawaljit1, Author
McDowell, Melanie A.2, Author                 
Gdowski, Courtney1, Author
Jo, Sunhwan1, Author
Wang, Yu1, Author
Benedict, Stephen H.1, Author
Lea, Susan M.1, Author
Galan, Jorge E.1, Author
De Guzman, Roberto N.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom, ou_persistent22              

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Free keywords: Bacterial Proteins, Bacterial Secretion Systems, Circular Dichroism, Nuclear Magnetic Resonance, Biomolecular, Protein Folding, Protein Structure, Secondary, Protein Transport, Salmonella typhimurium
 Abstract: The type III secretion system (T3SS) is essential in the pathogenesis of many bacteria. The inner rod is important in the assembly of the T3SS needle complex. However, the atomic structure of the inner rod protein is currently unknown. Based on computational methods, others have suggested that the Salmonella inner rod protein PrgJ is highly helical, forming a folded 3 helix structure. Here we show by CD and NMR spectroscopy that the monomeric form of PrgJ lacks a tertiary structure, and the only well-structured part of PrgJ is a short α-helix at the C-terminal region from residues 65-82. Disruption of this helix by glycine or proline mutation resulted in defective assembly of the needle complex, rendering bacteria incapable of secreting effector proteins. Likewise, CD and NMR data for the Shigella inner rod protein MxiI indicate this protein lacks a tertiary structure as well. Our results reveal that the monomeric forms of the T3SS inner rod proteins are partially folded.

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Language(s): eng - English
 Dates: 2012-05-312012-05-152012-05-312012-07-20
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1074/jbc.M112.381574
BibTex Citekey: zhong_salmonella_2012
 Degree: -

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Title: The Journal of Biological Chemistry
  Other : JBC
  Abbreviation : J. Biol. Chem.
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 287 (30) Sequence Number: - Start / End Page: 25303 - 25311 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1