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  Inside a shell – organometallic catalysis inside encapsulin nanoreactors

Lohner, P., Zmyslia, M., Thurn, J., Pape, J. K., Gerasimaite, R., Keller-Findeisen, J., et al. (2021). Inside a shell – organometallic catalysis inside encapsulin nanoreactors. Angewandte Chemie International Edition, 133(44), 24028-24034. doi:10.1002/ange.202110327.

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Lohner, P., Author
Zmyslia, M., Author
Thurn, J., Author
Pape, J. K.1, Author              
Gerasimaite, R.2, Author              
Keller-Findeisen, J.1, Author              
Groeer, S., Author
Deuringer, B., Author
Süss, R., Author
Walther, A., Author
Hell, S. W.1, Author              
Lukinavičius, G.2, Author              
Hugel, T., Author
Jessen-Trefzer, C., Author
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self-assembling protein-based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for intracellular compartmentalization of chemical reactions by design. Using single-molecule Förster resonance energy transfer and 3D-MINFLUX analysis, we analyze fluorescently labeled encapsulins on a single-molecule basis. Furthermore, by equipping these capsules with a synthetic ruthenium catalyst via covalent attachment to a non-native host protein, we are able to perform in vitro catalysis and go on to show that engineered encapsulins can be used as hosts for transition metal catalysis inside living cells in confined space.

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Language(s): eng - English
 Dates: 2021-08-212021-10-25
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/ange.202110327
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Title: Angewandte Chemie International Edition
Source Genre: Journal
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Pages: - Volume / Issue: 133 (44) Sequence Number: - Start / End Page: 24028 - 24034 Identifier: -