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  Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy

Butkevich, A. N., Weber, M., Delgado, A. R. C., Ostersehlt, L. M., D´Este, E., & Hell, S. W. (2021). Photoactivatable fluorescent dyes with hydrophilic caging groups and their use in multicolor nanoscopy. Journal of the American Chemical Society, 143(44), 18388-18393. doi:10.1021/jacs.1c09999.

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 Creators:
Butkevich, Alexey N.1, Author              
Weber, Michael, Author
Delgado, Angel R. Cereceda1, Author              
Ostersehlt, Lynn M., Author
D´Este, Elisa2, Author              
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Dyes and pigments, Peptides and proteins, Fluorescence, Microscopy, Fluorophores
 Abstract: We propose a series of fluorescent dyes with hydrophilic carbamate caging groups that undergo rapid photoactivation under UV (≤400 nm) irradiation but do not undergo spurious two-photon activation with high-intensity (visible or infrared) light of about twice the wavelength. The caged fluorescent dyes and labels derived therefrom display high water solubility and convert upon photoactivation into validated super-resolution and live-cell-compatible fluorophores. In combination with popular fluorescent markers, multiple (up to six)-color images can be obtained with stimulated emission depletion nanoscopy. Moreover, individual fluorophores can be localized with precision <3 nm (standard deviation) using MINSTED and MINFLUX techniques.

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Language(s): eng - English
 Dates: 2021-09-212021-10-292021-11-10
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of the American Chemical Society
  Other : JACS
  Abbreviation : J. Am. Chem. Soc.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 143 (44) Sequence Number: - Start / End Page: 18388 - 18393 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870