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  Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers

Wan, W., Clarke, M., Norris, M. J., Kolesnikova, L., Koehler, A., Bornholdt, Z. A., et al. (2020). Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers. Elife, 9: e59225. doi:10.7554/eLife.59225.

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 Creators:
Wan, W., Author
Clarke, M., Author
Norris, M. J., Author
Kolesnikova, L., Author
Koehler, A., Author
Bornholdt, Z. A., Author
Becker, S., Author
Saphire, E. O., Author
Briggs, John A. G.1, 2, Author           
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              
2MRC Laboratory of Molecular Biology, External Organizations, Cambridge, GB, ou_3346673              

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Free keywords: crystal-structure protein vp40 oligomerization visualization resolution nucleoprotein particles toolbox reveals system Life Sciences & Biomedicine - Other Topics
 Abstract: Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000586608800001
DOI: 10.7554/eLife.59225
ISSN: 2050-084X
 Degree: -

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Title: Elife
  Alternative Title : eLife
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 9 Sequence Number: e59225 Start / End Page: - Identifier: -