Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 
following Emergence of D614G Spike Variant

Mlcochova, P.; Collier, D.; Ritchie, A.; Assennato, S. M.; Hosmillo, M.; Goel, N.; Meng, B.; Chatterjee, K.; Mendoza, V.; Temperton, N.; Kiss, L.; James, L. C.; Ciazynska, K. A.; Xiong, X. L.; Briggs, John A. G.; Nathan, J. A.; Mescia, F.; Bergamaschi, L.; Zhang, H. Y.; Barmpounakis, P.; Demeris, N.; Skells, R.; Lyons, P. A.; Bradley, J.; Baker, S.; Allain, J. P.; Smith, K. G. C.; Bousfield, R.; Wilson, M.; Sparkes, D.; Amoroso, G.; Gkrania-Klotsas, E.; Hardwick, S.; Boyle, A.; Goodfellow, I.; Gupta, R. K.; Cambridge Inst Therapeutic, Immunol

doi:10.1016/j.xcrm.2020.100099

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Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

Mlcochova, P., Collier, D., Ritchie, A., Assennato, S. M., Hosmillo, M., Goel, N., et al. (2020). Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant. Cell Reports Medicine, 1(6): 100099. doi:10.1016/j.xcrm.2020.100099.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-723F-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-7240-E

Genre: Journal Article

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Creators:
Mlcochova, P., Author
Collier, D., Author
Ritchie, A., Author
Assennato, S. M., Author
Hosmillo, M., Author
Goel, N., Author
Meng, B., Author
Chatterjee, K., Author
Mendoza, V., Author
Temperton, N., Author
Kiss, L., Author
James, L. C., Author
Ciazynska, K. A., Author
Xiong, X. L., Author
Briggs, John A. G.¹, Author
Nathan, J. A., Author
Mescia, F., Author
Bergamaschi, L., Author
Zhang, H. Y., Author
Barmpounakis, P., Author
Demeris, N., AuthorSkells, R., AuthorLyons, P. A., AuthorBradley, J., AuthorBaker, S., AuthorAllain, J. P., AuthorSmith, K. G. C., AuthorBousfield, R., AuthorWilson, M., AuthorSparkes, D., AuthorAmoroso, G., AuthorGkrania-Klotsas, E., AuthorHardwick, S., AuthorBoyle, A., AuthorGoodfellow, I., AuthorGupta, R. K., AuthorCambridge Inst Therapeutic, Immunol, Author more..

Affiliations:
1MRC Laboratory of Molecular Biology, External Organizations, ou_3346673

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Free keywords: hospitalized-patients assay Cell Biology Research & Experimental Medicine

Abstract: Rapid COVID-19 diagnosis in the hospital is essential, although this is complicated by 30%-50% of nose/throat swabs being negative by SARS-CoV-2 nucleic acid amplification testing (NAAT). Furthermore, the D614G spike mutant dominates the pandemic and it is unclear how serological tests designed to detect anti-spike antibodies perform against this variant. We assess the diagnostic accuracy of combined rapid antibody point of care (POC) and nucleic acid assays for suspected COVID-19 disease due to either wildtype or the D614G spike mutant SARS-CoV-2. The overall detection rate for COVID-19 is 79.2% (95% CI 57.8-92.9) by rapid NAAT alone. The combined point of care antibody test and rapid NAAT is not affected by D614G and results in very high sensitivity for COVID-19 diagnosis with very high specificity.

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Language(s): eng - English

Dates: Date issued: 2020

Publication Status: Issued

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Identifiers: Other: WOS:000642300100009
DOI: 10.1016/j.xcrm.2020.100099
ISSN: 2666-3791

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Title: Cell Reports Medicine

Alternative Title : Cell Rep. Med.

Source Genre: Journal

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Pages: - Volume / Issue: 1 (6) Sequence Number: 100099 Start / End Page: - Identifier: -