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  The contributions of the actin machinery to endocytic membrane bending and vesicle formation

Picco, A., Kukulski, W., Manenschijn, H. E., Specht, T., Briggs, J. A. G., & Kaksonen, M. (2018). The contributions of the actin machinery to endocytic membrane bending and vesicle formation. Molecular Biology of the Cell, 29(11), 1346-1358. doi:10.1091/mbc.E17-11-0688.

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 Creators:
Picco, A., Author
Kukulski, W., Author
Manenschijn, H. E., Author
Specht, T., Author
Briggs, John A. G.1, 2, Author           
Kaksonen, M., Author
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              
2MRC Laboratory of Molecular Biology, External Organizations, Cambridge, GB, ou_3346673              

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Free keywords: clathrin-mediated endocytosis electron-microscopy fission yeast mechanical-properties plasma-membrane correlated fluorescence internalization step dynamin recruitment arp2/3 complex motor-activity Cell Biology
 Abstract: Branched and cross-linked actin networks mediate cellular processes that move and shape membranes. To understand how actin contributes during the different stages of endocytic membrane reshaping, we analyzed deletion mutants of yeast actin network components using a hybrid imaging approach that combines live imaging with correlative microscopy. We could thus temporally dissect the effects of different actin network perturbations, revealing distinct stages of actin-based membrane reshaping. Our data show that initiation of membrane bending requires the actin network to be physically linked to the plasma membrane and to be optimally cross-linked. Once initiated, the membrane invagination process is driven by nucleation and polymerization of new actin filaments, independent of the degree of cross-linking and unaffected by a surplus of actin network components. A key transition occurs 2 s before scission, when the filament nucleation rate drops. From that time point on, invagination growth and vesicle scission are driven by an expansion of the actin network without a proportional increase of net actin amounts. The expansion is sensitive to the amount of filamentous actin and its cross-linking. Our results suggest that the mechanism by which actin reshapes the membrane changes during the progress of endocytosis, possibly adapting to varying force requirements.

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Language(s): eng - English
 Dates: 2018
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000441051600009
DOI: 10.1091/mbc.E17-11-0688
ISSN: 1059-1524
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Title: Molecular Biology of the Cell
  Alternative Title : Mol. Biol. Cell
Source Genre: Journal
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Pages: - Volume / Issue: 29 (11) Sequence Number: - Start / End Page: 1346 - 1358 Identifier: -