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  9 angstrom structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments

Dodonova, S. O., Aderhold, P., Kopp, J., Ganeva, I., Rohling, S., Hagen, W. J. H., et al. (2017). 9 angstrom structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments. Elife, 6: e26691. doi:10.7554/eLife.26691.

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 Creators:
Dodonova, S. O., Author
Aderhold, P., Author
Kopp, J., Author
Ganeva, I., Author
Rohling, S., Author
Hagen, W. J. H., Author
Sinning, I., Author
Wieland, F., Author
Briggs, John A. G.1, 2, Author           
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              
2MRC Laboratory of Molecular Biology, External Organizations, Cambridge, GB, ou_3346673              

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Free keywords: ribosylation factor-i dilysine retrieval motifs structure refinement activating protein appendage domain complex reveals golgi recruitment transport subunit Life Sciences & Biomedicine - Other Topics
 Abstract: COPI coated vesicles mediate trafficking within the Golgi apparatus and between the Golgi and the endoplasmic reticulum. Assembly of a COPI coated vesicle is initiated by the small GTPase Arf1 that recruits the coatomer complex to the membrane, triggering polymerization and budding. The vesicle uncoats before fusion with a target membrane. Coat components are structurally conserved between COPI and clathrin/adaptor proteins. Using cryo-electron tomography and subtomogram averaging, we determined the structure of the COPI coat assembled on membranes in vitro at 9 angstrom resolution. We also obtained a 2.57 angstrom resolution crystal structure of beta delta-COP. By combining these structures we built a molecular model of the coat. We additionally determined the coat structure in the presence of ArfGAP proteins that regulate coat dissociation. We found that Arf1 occupies contrasting molecular environments within the coat, leading us to hypothesize that some Arf1 molecules may regulate vesicle assembly while others regulate coat disassembly.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000404026300001
DOI: 10.7554/eLife.26691
ISSN: 2050-084X
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Title: Elife
  Alternative Title : eLife
Source Genre: Journal
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Pages: - Volume / Issue: 6 Sequence Number: e26691 Start / End Page: - Identifier: -