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  The structure of the COPI coat determined within the cell

Bykov, Y. S., Schaffer, M., Dodonova, S. O., Albert, S., Plitzko, J. M., Baumeister, W., et al. (2017). The structure of the COPI coat determined within the cell. Elife, 6: e32493. doi:10.7554/eLife.32493.

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 Creators:
Bykov, Y. S., Author
Schaffer, M., Author
Dodonova, S. O., Author
Albert, S., Author
Plitzko, J. M., Author
Baumeister, W., Author
Engel, B. D., Author
Briggs, John A. G.1, 2, Author           
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              
2MRC Laboratory of Molecular Biology, External Organizations, Cambridge, GB, ou_3346673              

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Free keywords: particle electron cryomicroscopy golgi-apparatus cryoelectron tomography membrane-proteins alpha-cop vesicles complex transport arfgap1 motifs Life Sciences & Biomedicine - Other Topics
 Abstract: COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using purified components. Previously we have determined a complete structural model of the in vitro reconstituted COPI coat (Dodonova et al., 2017). Here, we applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determine the native structure of the COPI coat within vitrified Chlamydomonas reinhardtii cells. The native algal structure resembles the in vitro mammalian structure, but additionally reveals cargo bound beneath beta'-COP. We find that all coat components disassemble simultaneously and relatively rapidly after budding. Structural analysis in situ, maintaining Golgi topology, shows that vesicles change their size, membrane thickness, and cargo content as they progress from cis to trans, but the structure of the coat machinery remains constant.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000417124900001
DOI: 10.7554/eLife.32493
ISSN: 2050-084X
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Title: Elife
  Alternative Title : eLife
Source Genre: Journal
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Pages: - Volume / Issue: 6 Sequence Number: e32493 Start / End Page: - Identifier: -