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  Correlative light and electron microscopy methods for the study of virus-cell interactions

Bykov, Y. S., Cortese, M., Briggs, J. A. G., & Bartenschlager, R. (2016). Correlative light and electron microscopy methods for the study of virus-cell interactions. Febs Letters, 590(13), 1877-1895. doi:10.1002/1873-3468.12153.

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 Creators:
Bykov, Y. S., Author
Cortese, M., Author
Briggs, John A. G.1, Author           
Bartenschlager, R., Author
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              

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Free keywords: correlative light and electron microscopy electron microscopy virus-host interaction hepatitis-c virus semliki-forest-virus standard fluorescent proteins west-nile-virus cryoelectron tomography vitreous sections superresolution fluorescence localization microscopy multicellular organisms membranous replication Biochemistry & Molecular Biology Biophysics Cell Biology
 Abstract: Electron microscopy (EM) is an invaluable tool to study the interactions of viruses with cells, and the ultrastructural changes induced in host cells by virus infection. Light microscopy (LM) is a complementary tool with the potential to locate rare events, label specific components, and obtain dynamic information. The combination of LM and EM in correlative light and electron microscopy (CLEM) is particularly powerful. It can be used to complement a static EM image with dynamic data from live imaging, identify the ultrastructure observed in LM, or, conversely, provide molecular specificity data for a known ultrastructure. Here, we describe methods and strategies for CLEM, discuss their advantages and limitations, and review applications of CLEM to study virus-host interactions.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000380760800003
DOI: 10.1002/1873-3468.12153
ISSN: 1873-3468
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Title: Febs Letters
  Alternative Title : FEBS Lett.
Source Genre: Journal
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Pages: - Volume / Issue: 590 (13) Sequence Number: - Start / End Page: 1877 - 1895 Identifier: -