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  An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis

Skruzny, M., Desfosses, A., Prinz, S., Dodonova, S. O., Gieras, A., Uetrecht, C., et al. (2015). An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis. Developmental Cell, 33(2), 150-162. doi:10.1016/j.devcel.2015.02.023.

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 Creators:
Skruzny, M., Author
Desfosses, A., Author
Prinz, S., Author
Dodonova, S. O., Author
Gieras, A., Author
Uetrecht, C., Author
Jakobi, A. J., Author
Abella, M., Author
Hagen, W. J. H., Author
Schulz, J., Author
Meijers, R., Author
Rybin, V., Author
Briggs, John A. G.1, Author           
Sachse, C., Author
Kaksonen, M., Author
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              

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Free keywords: mediated endocytosis membrane curvature mass-spectrometry protein-structure plasma-membrane enth domain coated pits in-situ actin binding Cell Biology Developmental Biology
 Abstract: Clathrin-mediated endocytosis, the main trafficking route from the plasma membrane to the cytoplasm, is critical to many fundamental cellular processes. Clathrin, coupled to the membrane by adaptor proteins, is thought to play a major structural role in endocytosis by self-assembling into a cage-like lattice around the forming vesicle. Although clathrin adaptors are essential for endocytosis, little is known about their structural role in this process. Here we show that the membrane-binding domains of two conserved clathrin adaptors, Sla2 and Ent1, co-assemble in a PI(4,5)P-2-dependent manner to form organized lattices on membranes. We determined the structure of the co-assembled lattice by electron cryo-microscopy and designed mutations that specifically impair the lattice formation in vitro. We show that these mutations block endocytosis in vivo. We suggest that clathrin adaptors not only link the polymerized clathrin to the membrane but also form an oligomeric structure, which is essential for membrane remodeling during endocytosis.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000353352300006
DOI: 10.1016/j.devcel.2015.02.023
ISSN: 1534-5807
 Degree: -

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Title: Developmental Cell
  Alternative Title : Dev. Cell
Source Genre: Journal
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Pages: - Volume / Issue: 33 (2) Sequence Number: - Start / End Page: 150 - 162 Identifier: -