ausblenden:
Schlagwörter:
SNARE-mediated membrane fusion
cryo-electron microscopy
membrane
curvature
in vitro reconstitution
cryo-electron tomography
synaptic vesicles
synaptotagmin
complex
release
exocytosis
munc18-1
docking
Biochemistry & Molecular Biology
Cell Biology
Zusammenfassung:
Abstract Synaptic vesicles fuse with the plasma membrane in response to Ca2+ influx, thereby releasing neurotransmitters into the synaptic cleft. The protein machinery that mediates this process, consisting of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and regulatory proteins, is well known, but the mechanisms by which these proteins prime synaptic membranes for fusion are debated. In this study, we applied large-scale, automated cryo-electron tomography to image an in vitro system that reconstitutes synaptic fusion. Our findings suggest that upon docking and priming of vesicles for fast Ca-2(+)-triggered fusion, SNARE proteins act in concert with regulatory proteins to induce a local protrusion in the plasma membrane, directed towards the primed vesicle. The SNAREs and regulatory proteins thereby stabilize the membrane in a high-energy state from which the activation energy for fusion is profoundly reduced, allowing synchronous and instantaneous fusion upon release of the complexin clamp. Synopsis image Cryo-electron tomography of regulated SNARE-mediated membrane fusion shows that synaptic vesicles induce a protrusion in the target membrane that may prime them for fusion. Vesicles that are docked and primed for fusion in vitro are shown in an intermediate state characterized by a protrusion in the target membrane. High local membrane curvature of the protrusion may represent a high-energy state from which fast, coordinated fusion can occur upon Ca2+ activation.
