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  Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission

Beck, R., Prinz, S., Diestelkotter-Bachert, P., Rohling, S., Adolf, F., Hoehner, K., et al. (2011). Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission. Journal of Cell Biology, 194(5), 765-777. doi:10.1083/jcb.201011027.

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 Creators:
Beck, R., Author
Prinz, S., Author
Diestelkotter-Bachert, P., Author
Rohling, S., Author
Adolf, F., Author
Hoehner, K., Author
Welsch, S., Author
Ronchi, P., Author
Brugger, B., Author
Briggs, John A. G.1, Author           
Wieland, F., Author
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              

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Free keywords: gtp-dependent interaction coated vesicle formation endoplasmic-reticulum exchange factor copi vesicles protein curvature liposomes arfgap1 clathrin Cell Biology
 Abstract: Formation of coated vesicles requires two striking manipulations of the lipid bilayer. First, membrane curvature is induced to drive bud formation. Second, a scission reaction at the bud neck releases the vesicle. Using a reconstituted system for COPI vesicle formation from purified components, we find that a dimerization-deficient Arf1 mutant, which does not display the ability to modulate membrane curvature in vitro or to drive formation of coated vesicles, is able to recruit coatomer to allow formation of COPI-coated buds but does not support scission. Chemical cross-linking of this Arf1 mutant restores vesicle release. These experiments show that initial curvature of the bud is defined primarily by coatomer, whereas the membrane curvature modulating activity of dimeric Arf1 is required for membrane scission.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000294602500012
DOI: 10.1083/jcb.201011027
ISSN: 0021-9525
 Degree: -

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Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 194 (5) Sequence Number: - Start / End Page: 765 - 777 Identifier: -