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  Structural Analysis of HIV-1 Maturation Using Cryo-Electron Tomography

de Marco, A., Muller, B., Glass, B., Riches, J. D., Krausslich, H. G., & Briggs, J. A. G. (2010). Structural Analysis of HIV-1 Maturation Using Cryo-Electron Tomography. Plos Pathogens, 6(11): e1001215. doi:10.1371/journal.ppat.1001215.

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 Creators:
de Marco, A., Author
Muller, B., Author
Glass, B., Author
Riches, J. D., Author
Krausslich, H. G., Author
Briggs, John A. G.1, Author           
Affiliations:
1European Molecular Biology Laboratory, External Organizations, ou_3346677              

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Free keywords: immunodeficiency-virus type-1 rous-sarcoma-virus capsid protein virion maturation gag polyprotein cleavage sites in-vitro core visualization infectivity Microbiology Parasitology Virology
 Abstract: HIV-1 buds form infected cells in an immature, non-infectious form. Maturation into an infectious virion requires proteolytic cleavage of the Gag polyprotein at five positions, leading to a dramatic change in virus morphology. Immature virions contain an incomplete spherical shell where Gag is arranged with the N-terminal MA domain adjacent to the membrane, the CA domain adopting a hexameric lattice below the membrane, and beneath this, the NC domain and viral RNA forming a disordered layer. After maturation, NC and RNA are condensed within the particle surrounded by a conical CA core. Little is known about the sequence of structural changes that take place during maturation, however. Here we have used cryo-electron tomography and subtomogram averaging to resolve the structure of the Gag lattice in a panel of viruses containing point mutations abolishing cleavage at individual or multiple Gag cleavage sites. These studies describe the structural intermediates correlating with the ordered processing events that occur during the HIV-1 maturation process. After the first cleavage between SP1 and NC, the condensed NC-RNA may retain a link to the remaining Gag lattice. Initiation of disassembly of the immature Gag lattice requires cleavage to occur on both sides of CA-SP1, while assembly of the mature core also requires cleavage of SP1 from CA.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000284586300047
DOI: 10.1371/journal.ppat.1001215
ISSN: 1553-7366
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Title: Plos Pathogens
  Alternative Title : PLoS Pathog.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 6 (11) Sequence Number: e1001215 Start / End Page: - Identifier: -