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  Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ

Zanetti, G., Briggs, J. A. G., Grunewald, K., Sattentau, Q. J., & Fuller, S. D. (2006). Cryo-electron tomographic structure of an immunodeficiency virus envelope complex in situ. Plos Pathogens, 2(8): e83. doi:10.1371/journal.ppat.0020083.

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 Creators:
Zanetti, G., Author
Briggs, John A. G.1, Author           
Grunewald, K., Author
Sattentau, Q. J., Author
Fuller, S. D., Author
Affiliations:
1Ludwig-Maximilians-Universität München, External Organizations, Geschwister-Scholl-Platz 1, 80539 München, DE, ou_3346850              

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Free keywords: coreceptor-binding-site murine leukemia-virus monoclonal-antibodies receptor-binding 3-dimensional structure electron tomography glycoprotein gp120 type-1 hiv-1 neutralization Microbiology Parasitology Virology
 Abstract: The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for neutralizing antibodies. The receptor binding surfaces of Env are in large part sterically occluded or conformationally masked prior to receptor binding. Knowledge of the unliganded, trimeric Env structure is key for an understanding of viral entry and immune escape, and for the design of vaccines to elicit neutralizing antibodies. We have used cryo-electron tomography and averaging to obtain the structure of the SIV Env complex prior to fusion. Our result reveals novel details of Env organisation, including tight interaction between monomers in the gp41 trimer, associated with a three-lobed, membrane-distal gp120 trimer. A cavity exists at the gp41-gp120 trimer interface. Our model for the spike structure agrees with previously predicted interactions between gp41 monomers, and furthers our understanding of gp120 interactions within an intact spike.

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Language(s): eng - English
 Dates: 2006
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000202894800008
DOI: 10.1371/journal.ppat.0020083
ISSN: 1553-7374
 Degree: -

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Title: Plos Pathogens
  Alternative Title : PLoS Pathog.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 2 (8) Sequence Number: e83 Start / End Page: - Identifier: -