hide
Free keywords:
vesicular stomatitis-virus
human-immunodeficiency-virus
semliki-forest-virus
gpi-anchored proteins
human foamy virus
cryoelectron microscopy reveals
apical plasma-membrane
murine
leukemia-virus
canine kidney-cells
lipid rafts
Biotechnology & Applied Microbiology
Virology
Abstract:
Co-infection of a host cell by two unrelated enveloped viruses can lead to the production of pseudotypes: virions containing the genome of one virus but the envelope proteins of both viruses. The selection of components during virus assembly must therefore be flexible enough to allow the incorporation of unrelated viral membrane proteins, yet specific enough to exclude the bulk of host proteins. This apparent contradiction has been termed the pseudotypic paradox. There is mounting evidence that lipid rafts play a role in the assembly pathway of non-icosahedral, enveloped viruses. Viral components are concentrated initially in localized regions of the plasma membrane via their interaction with lipid raft domains. Lateral interactions of viral structural proteins amplify the changes in local lipid composition which in turn enhance the concentration of viral proteins in the rafts. An affinity for lipid rafts may be the common feature of enveloped virus proteins that leads to the formation of pseudotypes.
