English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Multiple site-specific infrared dichroism of CD3-zeta, a transmembrane helix bundle

Torres, J., Briggs, J. A. G., & Arkin, I. T. (2002). Multiple site-specific infrared dichroism of CD3-zeta, a transmembrane helix bundle. Journal of Molecular Biology, 316(2), 365-374. doi:10.1006/jmbi.2001.5267.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Torres, J., Author
Briggs, John A. G.1, Author           
Arkin, I. T., Author
Affiliations:
1University of Oxford, External Organizations, Oxford, UK, ou_3346844              

Content

show
hide
Free keywords: membrane proteins molecular dynamics molecular modelling site-specific infrared dichroism CD3-zeta zeta-chain dimerization secondary structure membrane-proteins orientation label model Biochemistry & Molecular Biology
 Abstract: The structure of the transmembrane domain of CD3-zeta a component of the T-cell receptor involved in signal transduction, has been studied in its native state (a lipid bilayer) by multiple site-specific infrared dichroism. For the first time, the transmembrane domain has been labelled at multiple positions along the sequence, representing a total of 11 samples, each labelled at a different residue with an isotopically modified carbonyl group, C-13=O-18. A strategy is outlined that, based on the above data, can yield the rotational orientation and the local helix tilt for each labelled residue, giving a detailed description of helix geometry. The results obtained indicate that the transmembrane segment is in an a-helical conformation throughout, with an average helix tilt of 12 degrees. The N-terminal side of the helix is more tilted than the C-terminal. In an accompanying paper we describe the implementation of the infrared data in a model-building study of the CD3-zeta transmembrane complex. The model obtained is entirely consistent with results based on evolutionary conservation data. Taken together, this study represents the first step towards elucidation of the backbone structure of a transmembrane a-helical bundle by infrared spectroscopy. (C)2002 Elsevier Science Ltd.

Details

show
hide
Language(s): eng - English
 Dates: 2002
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000174108100013
DOI: 10.1006/jmbi.2001.5267
ISSN: 0022-2836
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Molecular Biology
  Alternative Title : J. Mol. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 316 (2) Sequence Number: - Start / End Page: 365 - 374 Identifier: -