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  Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation

Ranjan, N., Pochopien, A. A., Wu, C.-C.-C., Beckert, B., Blanchet, S., Green, R., et al. (2021). Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation. EMBO Journal, 40(6): e106449. doi:10.15252/embj.2020106449.

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Ranjan, N.1, Author              
Pochopien, A. A., Author
Wu, C. C.-C., Author
Beckert, B., Author
Blanchet, S.1, Author              
Green, R., Author
Rodnina, M. V.2, Author              
Wilson, D. N., Author
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1Department of Physical Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578598              
2Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA–tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.

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Language(s): eng - English
 Dates: 2021-02-082021-03-15
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embj.2020106449
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Title: EMBO Journal
Source Genre: Journal
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Pages: 20 Volume / Issue: 40 (6) Sequence Number: e106449 Start / End Page: - Identifier: -